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Taken together, the results consolidate the fact that the human bone is not associated with the Pleistocene-age deposits of Darra-i-Kur; instead it is intrusive, possibly re-deposited from upper levels dating to much later periods (Neolithic).Despite its Holocene age, the Darra-i-Kur specimen is, so far, the first and only ancient human from Afghanistan whose DNA has been sequenced.DNA Bacteria Plus Kits are intended for molecular biology applications in food, animal feed, and pharmaceutical product testing.This product is not intended for the diagnosis, prevention, or treatment of a disease. For this Gram-positive bacterium, mechanical lysis delivered a more effective extraction, most evident at very low bacterial concentrations.His donor-dad is a non-enrolled admixed Cherokee, and there are stories on his maternal side of his grandmother’s romance with a Scots-Irish-Cherokee U. Army Air Forces bombardier during and after World War II. While the rank of any match is only relative to other matches and can’t tell you how much or what percentage of that ancestry you carry, a match to a population in the top 50 is of some significance.

Here we report the first direct radiocarbon date for the specimen and the genetic analyses of DNA extracted and sequenced from two areas of the bone.

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The new radiocarbon determination places the find to ∼4500 cal BP (∼2500 BCE) contradicting an assumed Palaeolithic age of ∼30,000 years, as originally suggested.

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